Fieldes M A, Tyson H
Biochem Genet. 1983 Apr;21(3-4):391-404. doi: 10.1007/BF00499147.
Anionic acid phosphatase (AP) isozymes in stem homogenates of flax genotrophs L and S were examined electrophoretically. The banding pattern was similar in both genotrophs. Relative mobility (Rm) was faster in each band of L than in the corresponding band of S. These Rm shifts did not result from differences in the sample concentration or from differential rates of development. AP activity was the same in both genotrophs. The banding pattern of F1 hybrids resembled the parental pattern. No multiple banding was observed in any of the F1 bands. The Rm in the F1 hybrids showed a dominance of L. Plots of loge (Rm) against gel concentration were constructed. While the Y intercepts of the plots indicated some differences in free electrophoretic mobility, molecular conformation was the major factor underlying both the Rm differences between bands and the Rm shifts between L and S, for corresponding bands. Estimates of apparent molecular weight (MW) for the different bands ranged from 25,000 to 250,000, with a strong possibility that some bands were oligomeric forms of others. Apparent MW was, on average, 17% higher in S than L.
用电泳法检测了亚麻基因型L和S茎匀浆中的阴离子酸性磷酸酶（AP）同工酶。两种基因型的条带模式相似。L型各条带的相对迁移率（Rm）比S型相应条带的迁移率快。这些Rm的变化不是由样品浓度差异或不同的显影速率引起的。两种基因型的AP活性相同。F1杂种的条带模式与亲本模式相似。在任何F1条带中均未观察到多条带。F1杂种中的Rm显示L型占优势。构建了loge（Rm）对凝胶浓度的图。虽然图的Y轴截距表明自由电泳迁移率存在一些差异，但分子构象是条带间Rm差异以及L和S型相应条带间Rm变化的主要因素。不同条带的表观分子量（MW）估计值在25,000至250,000之间，很有可能某些条带是其他条带的寡聚形式。S型的表观MW平均比L型高17%。
