Fieldes M A, Dixon B
Department of Biology, Wilfrid Laurier University, Waterloo, Ontario, Canada.
Biochem Genet. 1988 Apr;26(3-4):249-60. doi: 10.1007/BF00561464.
Ferguson plots demonstrated that corresponding malate dehydrogenase (MDH) isozymes of Durrant's L and S flax genotrophs differ in apparent molecular weight (MW) and also in net negative charge. The MW differences explain heritable differences in electrophoretic relative mobility (Rm) between corresponding L and S isozymes. The MW for each MDH isozyme was higher for L than for S and resulted in a slower Rm for L. The net negative charge for each isozyme was higher for L than for S. MDH isozymes also differ in MW within L and S. MW was lower for isozymes in leaves from the bottom of the stem than in leaves from the top of the stem, particularly in L. Integration of information on the MDH isozyme system in the flax genotrophs and information on the peroxidase system suggests the possibility that common modifier loci may control Rm in both enzymes.
弗格森作图表明，杜兰特的L型和S型亚麻基因型营养体中相应的苹果酸脱氢酶（MDH）同工酶在表观分子量（MW）和净负电荷方面存在差异。分子量差异解释了相应的L型和S型同工酶在电泳相对迁移率（Rm）上的遗传差异。每种MDH同工酶的分子量L型高于S型，导致L型的Rm较慢。每种同工酶的净负电荷L型高于S型。MDH同工酶在L型和S型内部的分子量也有所不同。茎基部叶片中的同工酶分子量低于茎顶部叶片中的同工酶，尤其是在L型中。将亚麻基因型营养体中MDH同工酶系统的信息与过氧化物酶系统的信息整合起来表明，可能存在共同的修饰基因座控制这两种酶的Rm。
