灌注糖尿病心脏中磷酸肌酸代谢异常。一项31P核磁共振研究。
Abnormal phosphocreatine metabolism in perfused diabetic hearts. A 31P nuclear-magnetic-resonance study.
作者信息
Pieper G M, Salhany J M, Murray W J, Wu S T, Eliot R S
出版信息
Biochem J. 1983 Feb 15;210(2):477-81. doi: 10.1042/bj2100477.
Abstract
31P n.m.r. analysis of control and diabetic hearts perfused for 1 h with a glucose buffer showed constant and normal levels of phosphocreatine and ATP. Supplementing the buffer with 0.5, 1.2 or 2.0 mM-palmitic acid had little or no effect on high-energy-phosphate levels in control hearts. In contrast, increases in palmitate concentration produced significant decreases in ATP in diabetic hearts, despite normal and constant levels of phosphocreatine. This 31P n.m.r. study suggests a defect in phosphocreatine metabolism in the perfused diabetic heart that might be related to creatine kinase kinetics.
摘要
用葡萄糖缓冲液灌注1小时的对照心脏和糖尿病心脏的31P核磁共振分析显示，磷酸肌酸和ATP水平恒定且正常。在缓冲液中添加0.5、1.2或2.0 mM的棕榈酸对对照心脏中的高能磷酸水平几乎没有影响。相比之下，尽管磷酸肌酸水平正常且恒定，但棕榈酸浓度的增加却使糖尿病心脏中的ATP显著降低。这项31P核磁共振研究表明，灌注的糖尿病心脏中磷酸肌酸代谢存在缺陷，这可能与肌酸激酶动力学有关。
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