Pacifico A, Cherchi G M, Deiana M G, Maioli M
Boll Soc Ital Biol Sper. 1983 Jan 31;59(2):199-205.
In order to further investigate plasma lipoproteins abnormalities secondary to serious liver damage, we studied plasma lipids and lipoproteins, and in particular HDL subfractions (HDL2, HDL3), in 12 patients with cirrhosis of the liver and in 12 sex, age and weight matched healthy volunteers. Enzymatic methods were used to determine total cholesterol and triglycerides, while the extractive method of Abell et al. was used for the determination of HDL-cholesterol levels after LDL and VLDL precipitation with polyanions (MnCl2 and Na-heparin) and of HDL3-cholesterol values after HDL2 precipitation with dextran-sulphate 15,000 m.w. Total cholesterol and HDL-cholesterol levels were significantly lower in cirrhotic patients compared to normal subjects. We must emphasize that only HDL3-cholesterol was decreased in cirrhotics, whereas HDL2-cholesterol values were normal or high. We suggest that a diminished activity of hepatic triglyceride lipase might account for the decrease in HDL3-cholesterol in liver cirrhosis.
为了进一步研究严重肝损伤继发的血浆脂蛋白异常，我们对12例肝硬化患者以及12名性别、年龄和体重相匹配的健康志愿者的血浆脂质和脂蛋白，特别是高密度脂蛋白亚组分（HDL2、HDL3）进行了研究。采用酶法测定总胆固醇和甘油三酯，而在使用聚阴离子（MnCl2和钠肝素）沉淀低密度脂蛋白和极低密度脂蛋白后，采用阿贝尔等人的萃取法测定高密度脂蛋白胆固醇水平；在使用分子量为15000的葡聚糖硫酸酯沉淀HDL2后，测定HDL3胆固醇值。与正常受试者相比，肝硬化患者的总胆固醇和高密度脂蛋白胆固醇水平显著降低。我们必须强调，肝硬化患者中只有HDL3胆固醇降低，而HDL2胆固醇值正常或升高。我们认为，肝甘油三酯脂肪酶活性降低可能是肝硬化患者HDL3胆固醇降低的原因。
