Gnasso A, Haberbosch W, Schettler G, Schmitz G, Augustin J
Klin Wochenschr. 1984;62 Suppl 2:36-42.
This study was designed to investigate acute effects of smoking on plasma lipoproteins. Twelve fasting smokers inhaled the smoke of two cigarettes. Blood samples were taken before and 30 min after smoking. Triglycerides decreased in plasma from 136.8 +/- 39.8 to 108.0 +/- 34.9, in VLDL from 79.8 +/- 31.4 to 58.4 +/- 28.5 mg%. Cholesterol fell in HDL3 from 29.9 +/- 5.0 to 26.6 +/- 5.8 mg%. Determination of HDL-cholesterol with the phosphotungstic acid precipitation method revealed a reduction from 45.7 +/- 10.2 to 40.3 +/- 11.1 mg% after smoking. All C-apoproteins decreased in VLDL as an entity. Apoprotein A-I was diminished in plasma from 137.5 +/- 23.1 to 118.2 +/- 25.4, and in HDL3, from 97.1 +/- 12.1 to 85.0 +/- 13.1, apoprotein A-II in plasma from 28.6 +/- 8.9 to 24.7 +/- 6.9 and in HDL3 from 21.1 +/- 8.4 to 17.4 +/- 5.7 mg%. Zonal ultracentrifugation of D less than 1.063 g/ml lipoproteins confirmed the influence of smoking on VLDL. In LDL, an increase in cholesterol esters, mainly cholesterol linoleate, from 57.3 +/- 30.2 to 67.4 +/- 25.9 mg% was observed after smoking. The HDL2 subclass was shifted toward a slightly lighter hydrated density, but the major effect of smoking was seen in HDL3. Total cholesterol decreased from 30.5 +/- 7.3 to 22.3 +/- 6.6 mg%, due to a fall of all major cholesterol esters from 23.7 +/- 7.2 to 15.8 +/- 6.9 mg%. HDL3-phospholipids were reduced from 74.9 +/- 37.0 to 46.0 +/- 23.9 mg%.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究旨在调查吸烟对血浆脂蛋白的急性影响。12名空腹吸烟者吸入两支香烟的烟雾。在吸烟前和吸烟后30分钟采集血样。血浆甘油三酯从136.8±39.8降至108.0±34.9，极低密度脂蛋白（VLDL）从79.8±31.4降至58.4±28.5mg%。高密度脂蛋白3（HDL3）中的胆固醇从29.9±5.0降至26.6±5.8mg%。用磷钨酸沉淀法测定高密度脂蛋白胆固醇显示，吸烟后从45.7±10.2降至40.3±11.1mg%。作为一个整体，VLDL中的所有C-载脂蛋白均减少。血浆中载脂蛋白A-I从137.5±23.1降至118.2±25.4，HDL3中从97.1±12.1降至85.0±13.1，血浆中载脂蛋白A-II从28.6±8.9降至24.7±6.9，HDL3中从21.1±8.4降至17.4±5.7mg%。对密度小于1.063g/ml的脂蛋白进行区带超速离心证实了吸烟对VLDL的影响。在低密度脂蛋白（LDL）中，吸烟后胆固醇酯增加，主要是胆固醇亚油酸酯，从57.3±30.2增至67.4±25.9mg%。HDL2亚类向稍轻的水合密度偏移，但吸烟的主要影响见于HDL3。总胆固醇从30.5±7.3降至22.3±6.6mg%，这是由于所有主要胆固醇酯从23.7±7.2降至15.8±6.9mg%。HDL3-磷脂从74.9±37.0降至46.0±23.9mg%。（摘要截取自250字）
