Marcel Y L, Vezina C, Milne R W
Biochim Biophys Acta. 1983 Feb 7;750(2):411-7. doi: 10.1016/0005-2760(83)90047-4.
The transfer of cholesteryl esters and apolipoprotein E has been studied between plasma HDL and chylomicrons isolated either from ascitic fluid or from the plasma of a patient with type V hyperlipoproteinemia. Whereas apolipoprotein E transfer was rapid and occurred at low temperature, cholesteryl ester transfer was suppressed at 4 degrees C. Apolipoprotein E transfer did not depend upon the presence of cholesteryl ester transfer protein and was in fact inhibited by the partially purified preparation of this protein. Apolipoprotein E transfer was not increased by reduction with dithiothreitol. The transfer of cholesteryl esters increased sharply at a chylomicron to HDL ratio of cholesteryl ester above 1/10, a value which may be of physiological significance at the peak of postprandial lipemia. At this ratio, the transfer of apolipoprotein E was minimal and increased only at ratios above 2/1. From these results, it is concluded that there is no connection between apolipoprotein E and cholesteryl ester transfer from HDL to chylomicrons. It is, therefore, proposed that whereas chylomicron apolipoprotein E is acquired rapidly and mostly in the lymphatic system, the concentration of chylomicron cholesteryl esters increases significantly and independently in the circulation.
已经对胆固醇酯和载脂蛋白E在血浆高密度脂蛋白（HDL）与从腹水或V型高脂蛋白血症患者血浆中分离出的乳糜微粒之间的转移进行了研究。载脂蛋白E的转移迅速且在低温下发生，而胆固醇酯的转移在4℃时受到抑制。载脂蛋白E的转移不依赖于胆固醇酯转移蛋白的存在，实际上还受到该蛋白部分纯化制剂的抑制。用二硫苏糖醇还原不会增加载脂蛋白E的转移。当乳糜微粒与HDL的胆固醇酯比例高于1/10时，胆固醇酯的转移急剧增加，该值在餐后血脂峰值时可能具有生理意义。在此比例下，载脂蛋白E的转移极少，仅在比例高于2/1时增加。从这些结果可以得出结论，载脂蛋白E与HDL向乳糜微粒的胆固醇酯转移之间没有关联。因此，有人提出，乳糜微粒载脂蛋白E在淋巴系统中快速获得且大部分如此，而乳糜微粒胆固醇酯的浓度在循环中显著且独立地增加。
