Stender S, Zilversmit D B
Arteriosclerosis. 1982 Nov-Dec;2(6):493-9. doi: 10.1161/01.atv.2.6.493.
Lymph chylomicrons labeled with 14C-cholesterol and plasma very low and intermediate density lipoproteins (d less than 1.019) labeled with 3H-cholesterol were injected simultaneously into recipient cholesterol-fed rabbits. The cholesteryl ester of chylomicron origin (14C) found in the tissues 1 to 4 hours after the injection is the sum of both direct and indirect uptake of chylomicron cholesteryl ester. The direct uptake of chylomicron cholesteryl ester is defined as occurring in situ during the interaction between chylomicrons and lipoprotein lipase in endothelial cells. The indirect uptake of chylomicron cholesteryl ester results from the uptake of chylomicron remnants and of other plasma lipoproteins which become labeled during the degradation of the injected chylomicrons by net lipid transfer or by exchange. The indirect contribution of chylomicron cholesteryl ester to tissues was calculated from tissue 3H-cholesteryl ester derived from injected plasma d less than 1.019 lipoproteins. In rabbits fed 0.5 g cholesterol daily for 40-60 days, with plasma cholesterol concentrations between 20 and 30 mM (800-1200 mg/dl), less than 1% of the cholesteryl ester influx into aortic intima media and less than 10% in liver was derived from the direct uptake of chylomicron cholesteryl ester.
将用¹⁴C - 胆固醇标记的淋巴乳糜微粒和用³H - 胆固醇标记的血浆极低密度和中间密度脂蛋白（d小于1.019）同时注射到接受胆固醇喂养的兔子体内。注射后1至4小时在组织中发现的乳糜微粒来源的胆固醇酯（¹⁴C）是乳糜微粒胆固醇酯直接摄取和间接摄取的总和。乳糜微粒胆固醇酯的直接摄取定义为在乳糜微粒与内皮细胞中的脂蛋白脂肪酶相互作用期间在原位发生。乳糜微粒胆固醇酯的间接摄取是由于摄取乳糜微粒残粒和其他血浆脂蛋白所致，这些脂蛋白在注射的乳糜微粒通过净脂质转移或交换降解过程中被标记。乳糜微粒胆固醇酯对组织的间接贡献是根据源自注射的血浆d小于1.019脂蛋白的组织³H - 胆固醇酯计算得出的。在每天喂食0.5 g胆固醇持续40 - 60天、血浆胆固醇浓度在20至30 mM（800 - 1200 mg/dl）之间的兔子中，流入主动脉内膜中层的胆固醇酯中来自乳糜微粒胆固醇酯直接摄取的不到1%，在肝脏中不到10%。
