Gourley G R, Mogilevsky W, Odell G B
Biochim Biophys Acta. 1983 Feb 7;750(2):419-23. doi: 10.1016/0005-2760(83)90048-6.
The homozygous jaundiced (jj) Gunn rat exhibits hepatic microsomal enzyme activities which vary from markedly decreased to normal when compared with the non-jaundiced (JJ) Gunn rat. In order to determine if an alteration in microsomal lipid might be related to these observations, cholesterol, phospholipid, fatty acid and fluorescence polarization determinations were carried out in Gunn rats of both genotypes. Significant differences in microsomal palmitic, stearic and arachidonic acid composition were present, but these were not striking. Fluorescence polarization data best fit a two-phase linear model for both genotypes with no significant differences in breakpoint temperatures. In jj rats, the anisotrophy parameter ((r0/r)-1)-1 was significantly greater than that seen in JJ rats at both 25 and 37 degrees C, indicating a decreased membrane fluidity in the jaundiced animals. Alterations in enzyme microenvironment due to subtle changes in lipid composition may be related to the different enzyme activities observed in Gunn rats.
纯合黄疸型（jj）冈恩大鼠的肝微粒体酶活性与非黄疸型（JJ）冈恩大鼠相比，从显著降低到正常不等。为了确定微粒体脂质的改变是否可能与这些观察结果相关，对两种基因型的冈恩大鼠进行了胆固醇、磷脂、脂肪酸和荧光偏振测定。微粒体棕榈酸、硬脂酸和花生四烯酸组成存在显著差异，但并不显著。荧光偏振数据最符合两种基因型的双相线性模型，断点温度无显著差异。在jj大鼠中，各向异性参数（(r0/r)-1)-1在25℃和37℃时均显著高于JJ大鼠，表明黄疸动物的膜流动性降低。脂质组成的细微变化导致的酶微环境改变可能与冈恩大鼠中观察到的不同酶活性有关。
