Kopachik W, Oohata A, Dhokia B, Brookman J J, Kay R R
Cell. 1983 Jun;33(2):397-403. doi: 10.1016/0092-8674(83)90421-x.
DIF is an endogenous extracellular signal that may control differentiation of D. discoideum cells. It is a dialyzable, lipid-like factor that induces stalk cell formation among isolated amebae incubated in vitro with cAMP. To examine the consequences of DIF deprivation, we have isolated several mutant strains that are impaired in DIF accumulation, and whose inability to make stalk cells in vitro and during normal development on agar can be corrected by the addition of exogenous DIF. Little DIF is made by the mutants, and morphological development on agar stops after the cells have aggregated, but before a slug forms. In these DIF-deprived conditions, prespore cells can differentiate, but prestalk cells cannot.
DIF是一种内源性细胞外信号，可能控制盘基网柄菌细胞的分化。它是一种可透析的、类脂因子，能在体外与cAMP一起孵育的分离变形虫中诱导柄细胞形成。为了研究DIF缺失的后果，我们分离了几种在DIF积累方面受损的突变菌株，它们在体外和琼脂上正常发育过程中无法形成柄细胞的情况可通过添加外源性DIF来纠正。这些突变体产生的DIF很少，在琼脂上的形态发育在细胞聚集后但在蛞蝓体形成之前就停止了。在这些DIF缺失的条件下，前孢子细胞可以分化，但前柄细胞不能。
