Mak N K, Schiltknecht E, Ada G L
Cell Immunol. 1983 Jun;78(2):314-25. doi: 10.1016/0008-8749(83)90286-1.
Groups of C57BL/6J, BALB/c, BALB/c, nu+/nu+ mice, inoculated intranasally with Corynebacterium parvum (350 micrograms/mouse) were protected from death by an otherwise lethal dose of influenza virus, A/WSN (H1N1) inoculated 3 days later. The lungs of C. parvum-treated, virus-infected C57BL/6J, BALB/c, or BALB/c nu+/nu+ mice contained significantly less infectious virus than did controls, and this reduction was apparent as soon as 24 hr after virus inoculation. The maximum protective effect correlated with increased lung interferon levels. C. parvum treatment caused an increase in the lung cell number which was in part due to a large increase (ca. 10-fold) in macrophage content, and the natural killer cell activity was also enhanced, though not as markedly as occurred 3 days after infection. Most (greater than 85%) of the resident macrophages in normal lungs were susceptible to infection by virus (as indicated by hemadsorption), whereas most of those recovered from the lungs of C. parvum-treated mice resisted infection. Despite the increase in macrophage content, the level of specific immune responses to infection, such as cytotoxic T-cell activity, DTH reaction, and antihemagglutinin antibody, remained unchanged by C. parvum treatment so that the major if not only effect of this treatment was on the level of the less-specific components of the immune system.
给C57BL/6J、BALB/c、BALB/c裸鼠（nu+/nu+）经鼻接种微小棒状杆菌（350微克/只小鼠），3天后接种致死剂量的甲型流感病毒A/WSN（H1N1），这些小鼠可免于死亡。经微小棒状杆菌处理且感染病毒的C57BL/6J、BALB/c或BALB/c裸鼠肺中含有的感染性病毒明显少于对照组，且在病毒接种后24小时即明显减少。最大保护作用与肺中干扰素水平升高相关。微小棒状杆菌处理导致肺细胞数量增加，部分原因是巨噬细胞含量大幅增加（约10倍），自然杀伤细胞活性也增强，尽管不如感染后3天增强明显。正常肺中大多数（超过85%）驻留巨噬细胞易被病毒感染（通过血细胞吸附显示），而从经微小棒状杆菌处理的小鼠肺中回收的大多数巨噬细胞抵抗感染。尽管巨噬细胞含量增加，但微小棒状杆菌处理对感染的特异性免疫反应水平，如细胞毒性T细胞活性、迟发型超敏反应和抗血凝素抗体，并无改变，因此该处理的主要（如果不是唯一）作用是作用于免疫系统非特异性成分的水平。
