Jones B L, Mak A S
Eur J Biochem. 1983 Jul 1;133(3):621-5. doi: 10.1111/j.1432-1033.1983.tb07508.x.
Purothionins were extracted and purified from the diploid wheat Triticum monococcum. Two proteins were obtained, one of which was present in only very small amounts. The major purothionin of T. monococcum was sequenced and it had an amino acid sequence identical with that of the beta-purothionin of Triticum aestivum (hexaploid bread wheat). It is known that T. monococcum contains the wheat A genome, so the structural gene coding for the beta-purothionin must comprise a part of the A genome. There have been no observable (as amino acid replacements) changes in the DNA comprising either the beta-purothionin gene of T. aestivum or the purothionin gene of T. monococcum, since T. monococcum (or its wild equivalent, Triticum boeoticum) hybridized with the diploid wheat B genome progenitor and started the evolution from diploid to allohexaploid wheat. All of the investigated characteristics of the purothionin-like protein isolated in small amounts suggested that it was essentially identical in amino acid sequence with the T. monococcum purothionin. It may be a dimerized form of beta-purothionin.
从二倍体小麦一粒小麦中提取并纯化了硫堇蛋白。得到了两种蛋白质，其中一种的含量极少。对一粒小麦的主要硫堇蛋白进行了测序，其氨基酸序列与普通小麦（六倍体面包小麦）的β-硫堇蛋白相同。已知一粒小麦含有小麦A基因组，因此编码β-硫堇蛋白的结构基因必定是A基因组的一部分。自一粒小麦（或其野生近缘种野生一粒小麦）与二倍体小麦B基因组祖先杂交并开启从二倍体小麦到异源六倍体小麦的进化以来，构成普通小麦β-硫堇蛋白基因或一粒小麦硫堇蛋白基因的DNA没有观察到（作为氨基酸替换的）变化。少量分离得到的类硫堇蛋白的所有研究特征表明，其氨基酸序列与一粒小麦硫堇蛋白基本相同。它可能是β-硫堇蛋白的二聚体形式。
