快速纯化含有内源性激酶活性的牛肾支链2-氧代酸脱氢酶复合物。
Rapid purification of bovine kidney branched-chain 2-oxoacid dehydrogenase complex containing endogenous kinase activity.
作者信息
Lawson R, Cook K G, Yeaman S J
出版信息
FEBS Lett. 1983 Jun 27;157(1):54-8. doi: 10.1016/0014-5793(83)81115-6.
PMID:6862020
Abstract
Branched-chain 2-oxoacid dehydrogenase complex has been purified to near homogeneity by a simple, rapid procedure. The final product contains endogenous kinase activity capable of phosphorylating and inactivating the complex. Phosphorylation continues after complete inactivation, indicating the possibility of several phosphorylatable sites.
摘要
支链2-氧代酸脱氢酶复合体已通过一种简单、快速的方法纯化至近乎同质。最终产物含有能够使复合体磷酸化并使其失活的内源性激酶活性。在完全失活后磷酸化仍在继续，这表明存在多个可磷酸化位点的可能性。
Zotero 插件