Kaneko Y, Obara T, Fujimoto Y
Horm Metab Res. 1983 Apr;15(4):187-91. doi: 10.1055/s-2007-1018665.
Medullary thyroid carcinomas (MCT) were obtained from patients with Sipple's syndrome or non-hereditary MCT. Cultures of enzymatically dissociated cells showed that MCT cells were mostly free-floating cells of epithelial characters. These MCT cells preserved the ability to secrete calcitonin for a long period of in vitro maintenance. However, the MCT cells under the usual culture conditions were filled with numerous cytoplasmic granules and appeared to be extremely fragile. When MCT cells were cultured in a low-calcium medium, such granules were not observed. In addition, MCT cells in the low-calcium medium increased the amount of calcitonin in response to added calcium and tetragastrin. Therefore, the culture of MCT cells in the low-calcium medium appeared to be useful for studies about the biochemical and physiological characters of human MCT cells in vitro.
甲状腺髓样癌（MCT）取自患有多发性内分泌腺瘤2型综合征（Sipple综合征）的患者或非遗传性MCT患者。酶解细胞培养显示，MCT细胞大多是具有上皮特征的悬浮细胞。这些MCT细胞在长时间体外培养中保留了分泌降钙素的能力。然而，在常规培养条件下的MCT细胞充满了大量细胞质颗粒，看起来极其脆弱。当MCT细胞在低钙培养基中培养时，未观察到此类颗粒。此外，低钙培养基中的MCT细胞对添加的钙和胃泌素四肽有反应，降钙素分泌量增加。因此，在低钙培养基中培养MCT细胞似乎有助于体外研究人MCT细胞的生化和生理特性。
