Rangachari P K, Grover A K, Daniel E E
Hypertension. 1983 Jul-Aug;5(4):436-41. doi: 10.1161/01.hyp.5.4.436.
Most of the thallium uptake by canine iliac arteries (70%) was inhibited by ouabain. The component sensitive to ouabain was significantly increased by incubating tissues in K+-free solution and reduced by low temperatures, metabolic inhibitors, and loading with lithium. The apparent Km for thallium for the ouabain-sensitive uptake was 0.47 +/- 0.046 mM. External K+ inhibited thallium uptake and the apparent Ki was estimated to be 6.5 mM. Thallium thus appears to have a greater affinity for the ouabain-sensitive components. The residual component of thallium uptake was unaffected by maneuvers that affected the ouabain-sensitive uptake, which thus appears to be a suitable indicator of Na+ pump activity in vascular smooth muscle.
犬髂动脉对铊的摄取大部分（70%）被哇巴因抑制。将组织置于无钾溶液中孵育可使对哇巴因敏感的成分显著增加，而低温、代谢抑制剂以及锂负荷则使其减少。对哇巴因敏感的铊摄取的表观Km为0.47±0.046 mM。细胞外钾抑制铊摄取，表观Ki估计为6.5 mM。因此，铊对哇巴因敏感成分似乎具有更高的亲和力。铊摄取的残余成分不受影响哇巴因敏感摄取的操作的影响，因此，哇巴因敏感摄取似乎是血管平滑肌中钠泵活性的合适指标。
