Babu J P, Simpson W A, Courtney H S, Beachey E H
Infect Immun. 1983 Jul;41(1):162-8. doi: 10.1128/iai.41.1.162-168.1983.
The interaction of purified human plasma fibronectin (Fn) with bacteria was studied with a variety of oral streptococci. Each of the strains of Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguis, and Streptococcus mitis tested was aggregated by Fn to various degrees, depending on the concentration of Fn added to the test mixtures. Binding assays performed with radiolabeled Fn and various strains of streptococci demonstrated various capabilities to bind Fn, and the amount of Fn bound by each strain was paralleled by its Fn-induced aggregation, with S. mutans 6715 giving the highest values in both assays. Because of the avid binding of Fn by certain strains of potentially cariogenic streptococci, we investigated the possibility that Fn may be present in human saliva and may be adsorbed from saliva onto artificial tooth pellicles. Immunoreactive Fn was detected in paraffin-stimulated whole saliva by enzyme-linked immunosorbent assays of saliva adsorbed onto gelatin-coated cuvettes and by immunoelectroblots (Western blots) of salivary components separated on sodium dodecyl sulfate-polyacrylamide slab gels. Furthermore, immunoreactive Fn was found to be present in artificial tooth pellicles formed by incubating hydroxyapatite beads with whole human saliva. These results demonstrate that certain strains of oral streptococci bind to and are aggregated by Fn. The presence of Fn in artificial tooth pellicles suggests that this macromolecule may play a role in the attachment of potentially cariogenic and other oral streptococci to dental tissues.
利用多种口腔链球菌研究了纯化的人血浆纤连蛋白（Fn）与细菌的相互作用。变形链球菌、唾液链球菌、血链球菌和缓症链球菌的每个测试菌株都被Fn不同程度地聚集，这取决于添加到测试混合物中的Fn浓度。用放射性标记的Fn和各种链球菌菌株进行的结合试验表明，它们结合Fn的能力各不相同，每个菌株结合的Fn量与其由Fn诱导的聚集程度平行，在这两种试验中变形链球菌6715的值最高。由于某些潜在致龋性链球菌菌株对Fn有强烈的结合作用，我们研究了Fn可能存在于人类唾液中并可能从唾液吸附到人工牙菌斑上的可能性。通过酶联免疫吸附测定法检测吸附在明胶包被比色皿上的唾液，以及通过对在十二烷基硫酸钠-聚丙烯酰胺平板凝胶上分离的唾液成分进行免疫印迹（Western印迹），在石蜡刺激的全唾液中检测到了免疫反应性Fn。此外，通过将羟基磷灰石珠与全人类唾液孵育形成的人工牙菌斑中也发现了免疫反应性Fn。这些结果表明，某些口腔链球菌菌株能与Fn结合并被其聚集。人工牙菌斑中存在Fn表明，这种大分子可能在潜在致龋性链球菌和其他口腔链球菌附着于牙齿组织的过程中发挥作用。
