Ying W, Hedman M, de la Torre B, Jensen F, Pedersen P H, Diczfalusy E
Int J Androl. 1983 Apr;6(2):116-24. doi: 10.1111/j.1365-2605.1983.tb00330.x.
The effect of vasectomy on the steroid profile of seminal plasma was studied in 19 healthy men at 4-monthly intervals during a 2-year post-vasectomy period. The steroids analyzed by radioimmunoassays were: androstenedione (A), testosterone (T), dihydrotestosterone (DHT), oestradiol (E2) and the sulphoconjugated forms of pregnenolone (delta 5P-S), dehydroepiandrosterone (DHEA-S), testosterone (T-S), dihydrotestosterone (DHT-S), oestrone (E1-S) and oestradiol (E2-S). The quantitatively most important steroids present in the seminal plasma were DHEA-S, delta 5P-S and DHT-S. Vasectomy resulted in a significant but non-progressive reduction in DHT (40%) and T (23%) levels. The levels of E2, delta 5P-S and DHT-S also exhibited a smaller reduction following vasectomy. No significant changes were observed in the levels of the other steroids studied. The results are interpreted as suggesting that under normal conditions the bulk of the steroids present in the ejaculate is contributed by the accessory reproductive organs. In addition, a significant part of the DHT and T reach the ejaculate together with the sperm directly from the testis and/or epididymis. It is suggested that the normal steroid levels in seminal plasma established in this investigation could serve as reference values in the hormonal evaluation of male infertility.
在输精管结扎术后2年期间，对19名健康男性每隔4个月进行一次研究，以观察输精管结扎对精浆类固醇谱的影响。通过放射免疫分析法分析的类固醇包括：雄烯二酮（A）、睾酮（T）、双氢睾酮（DHT）、雌二醇（E2）以及孕烯醇酮（δ5P-S）、脱氢表雄酮（DHEA-S）、睾酮（T-S）、双氢睾酮（DHT-S）、雌酮（E1-S）和雌二醇（E2-S）的硫酸共轭形式。精浆中含量最丰富的类固醇是DHEA-S、δ5P-S和DHT-S。输精管结扎导致DHT（40%）和T（23%）水平显著但非进行性降低。输精管结扎后，E2、δ5P-S和DHT-S水平也有较小程度的降低。在所研究的其他类固醇水平上未观察到显著变化。结果表明，在正常情况下，射精中存在的大部分类固醇由附属生殖器官提供。此外，相当一部分DHT和T与精子一起直接从睾丸和/或附睾进入射精。建议本研究中建立的精浆正常类固醇水平可作为男性不育症激素评估的参考值。
