Aitken R J, Liu J, Best F S, Richardson D W
Int J Androl. 1983 Apr;6(2):157-67. doi: 10.1111/j.1365-2605.1983.tb00334.x.
The direct effects of gossypol and its acetic acid adduct, on the movement and functional competence of human spermatozoa were investigated employing exposure times of 1, 5 or 15 min and concentrations of 50 microM, 500 microM and 1000 microM. These compounds markedly reduced the motility, velocity, frequency of sperm head rotation and linearity of sperm progression, the most significant effects being observed with gossypol acetic acid on populations of 'capacitated' spermatozoa. Significant direct effects of gossypol on the ability of human spermatozoa to penetrate both cervical mucus and zona-free hamster ova were also observed, which were independent of any effects on motility. These results reinforce the notion that gossypol may serve a contraceptive role in the female as a 'spermicidal' agent, and suggest that this compound may also be of scientific value as a probe for identifying and isolating functionally important components of the human spermatozoon.
采用1、5或15分钟的暴露时间以及50微摩尔、500微摩尔和1000微摩尔的浓度，研究了棉酚及其醋酸加成物对人类精子运动和功能活性的直接影响。这些化合物显著降低了精子的活力、速度、精子头部旋转频率和精子前进的直线性，棉酚醋酸对“获能”精子群体的影响最为显著。还观察到棉酚对人类精子穿透宫颈黏液和去透明带仓鼠卵的能力有显著直接影响，且这些影响与对活力的任何影响无关。这些结果强化了棉酚可能作为一种“杀精剂”在女性中起到避孕作用的观念，并表明该化合物作为识别和分离人类精子功能重要成分的探针可能也具有科学价值。
