Rapaka R S, Okamoto K, Long M M, Urry D W
Int J Pept Protein Res. 1983 Apr;21(4):352-63.
Synthesis of two copolymers, H-(phi-Pro-Gly-Gly)n-Val-OMe and H-(phi'-Pro-Gly-Gly)n-Val-OMe, where phi is Val or Lys and phi' is Val or Glu is described. Cross-linking between the two copolymers is achieved by a coupling reaction between the epsilon-amino groups of the lysine containing copolymer and delta-carboxyls of the glutamic acid of the other copolymer. The cross-linking reaction was performed during a temperature elicited phase separation with flow orientation of the copolymers. Coacervation of the intermediate polymers is presented, as is the scanning electron micrograph of the insoluble cross-linked product and its calcifiability as determined with electron probe microanalysis. The purity of the key intermediates and polymers is demonstrated by the usual analytical methods. Carbon-13 magnetic resonance spectra of the intermediate monomer units are included to validate the purity of their synthesis.
描述了两种共聚物H-(φ-脯氨酸-甘氨酸-甘氨酸)n-缬氨酸-甲氧基和H-(φ'-脯氨酸-甘氨酸-甘氨酸)n-缬氨酸-甲氧基的合成，其中φ为缬氨酸或赖氨酸，φ'为缬氨酸或谷氨酸。两种共聚物之间的交联是通过含赖氨酸共聚物的ε-氨基与另一种共聚物谷氨酸的δ-羧基之间的偶联反应实现的。交联反应在温度引发的相分离过程中进行，共聚物具有流动取向。介绍了中间聚合物的凝聚现象，以及不溶性交联产物的扫描电子显微镜照片及其通过电子探针微分析测定的钙化能力。通过常规分析方法证明了关键中间体和聚合物的纯度。还包括中间单体单元的碳-13磁共振光谱，以验证其合成的纯度。
