弹性蛋白连续多肽模型中的凝聚特性。H-(丙氨酸-脯氨酸-甘氨酸-甘氨酸)n-缬氨酸甲酯和H-(丙氨酸-脯氨酸-甘氨酸-缬氨酸-甘氨酸)n-缬氨酸甲酯的合成。

Coacervation properties in sequential polypeptide models of elastin. Synthesis of H-(Ala-Pro-Gly-Gly)n-Val-OMe and H-(Ala-Pro-Gly-Val-Gly)n-Val-OMe.

作者信息

Rapaka R S, Okamoto K, Urry D W

出版信息

Int J Pept Protein Res. 1978 Aug;12(2):81-92. doi: 10.1111/j.1399-3011.1978.tb02871.x.

DOI:10.1111/j.1399-3011.1978.tb02871.x

Abstract

Syntheses of two sequential polypeptides H-(Ala-Pro-Gly-Gly)n-Val-OMe and H-(Ala-Pro-Gly-Val-Gly)n-Val-OMe via the p-nitrophenyl active ester procedure are reported. The two polymers were obtained in good yields and the polymers were shown to be of large molecular weights, n greater than 40. These two polypeptides were synthesized as analogs of the two coacervating sequential polypeptides H-(Val-Pro-Gly-Gly)n-Val-OMe, and H-(Val-Pro-Gly-Val-Gly)n-Val-OMe, in which the Val-l residue is replaced by an Ala-l residue. H-(Ala-Pro-Gly-Gly)n-Val-OMe did not coacervate even at as high a temperature as 100 degrees, and H-(Ala-Pro-Gly-Val-Gly)n-Val-OMe did not coacervate; however, it precipitated irreversibly around 65--70 degrees C. This suggests the critical role of the Val-Pro hydrophobic side chain interaction in coacervation.

摘要

报道了通过对硝基苯基活性酯法合成两种序列多肽H-(Ala-Pro-Gly-Gly)n-Val-OMe和H-(Ala-Pro-Gly-Val-Gly)n-Val-OMe。这两种聚合物产率良好，且显示出高分子量，n大于40。这两种多肽是作为两种凝聚序列多肽H-(Val-Pro-Gly-Gly)n-Val-OMe和H-(Val-Pro-Gly-Val-Gly)n-Val-OMe的类似物合成的，其中Val-1残基被Ala-1残基取代。H-(Ala-Pro-Gly-Gly)n-Val-OMe即使在高达100℃的温度下也不会凝聚，H-(Ala-Pro-Gly-Val-Gly)n-Val-OMe也不会凝聚；然而，它在65-70℃左右不可逆地沉淀。这表明Val-Pro疏水侧链相互作用在凝聚过程中起关键作用。