Madtes P, Redburn D A
Invest Ophthalmol Vis Sci. 1983 Jul;24(7):886-92.
A procedure by which the activity of the retinal GABA uptake system can be manipulated in vivo has been developed. Intraocular injections of nipecotic acid, a proported GABA uptake blocker, were administered to adult rabbits every 48 hours for a two-week period. No behavioral or systemic changes were observed. Injections were well-tolerated with less than 10% loss of the tissue caused by physical damage or injection. Biochemical analyses demonstrated a dose-dependent inhibition of 14C-GABA uptake into retinal tissue. No effect on uptake was observed for saline-treated tissue. Autoradiographic analyses showed that in vivo treatment with nipecotic acid preferentially blocked accumulation of 3H-GABA into the amacrine cell bodies and processes in the inner plexiform layer. This treatment may be especially useful in assessing the functional significance of GABA transport in vivo.
已开发出一种可在体内操纵视网膜GABA摄取系统活性的方法。在两周的时间里，每隔48小时给成年兔子眼内注射哌啶酸（一种据称的GABA摄取阻滞剂）。未观察到行为或全身变化。注射耐受性良好，因物理损伤或注射导致的组织损失不到10%。生化分析表明，14C-GABA摄取到视网膜组织中受到剂量依赖性抑制。用盐水处理的组织未观察到对摄取的影响。放射自显影分析表明，体内用哌啶酸处理可优先阻止3H-GABA在内网状层无长突细胞体和突起中的积累。这种处理在评估体内GABA转运的功能意义方面可能特别有用。
