弗兰克氏菌属中的质粒
Plasmids in Frankia sp.
作者信息
Normand P, Simonet P, Butour J L, Rosenberg C, Moiroud A, Lalonde M
出版信息
J Bacteriol. 1983 Jul;155(1):32-5. doi: 10.1128/jb.155.1.32-35.1983.
A method to achieve cell lysis and isolate Frankia sp. plasmid DNA was developed. A screening of Frankia sp. strains belonging to different host compatibility groups (Alnus sp., Elaeagnus sp., Ceanothus sp.) showed that, of 39 strains tested, 4 (strains Cp11, ARgN22d, ArI3, and EUN1f) possessed plasmids ranging in size from 7.1 to 32.2 kilobase pairs as estimated from agarose gel electrophoresis and electron microscopy. A total of 11 plasmids were detected.
开发了一种实现细胞裂解并分离弗兰克氏菌属质粒DNA的方法。对属于不同宿主相容性组（桤木属、胡颓子属、鼠李属）的弗兰克氏菌属菌株进行筛选，结果表明，在所测试的39个菌株中，有4个（菌株Cp11、ARgN22d、ArI3和EUN1f）拥有质粒，根据琼脂糖凝胶电泳和电子显微镜估计，其大小在7.1至32.2千碱基对之间。总共检测到11个质粒。
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