钴原卟啉IX可防止血清对细胞黏附的抑制作用。

The inhibitory effect of serum on cell attachment can be prevented by cobalt-protoporphyrin IX.

作者信息

Verger C, Imbenotte J

机构信息

ER 303 C.N.R.S., I.R.S.C., Villejuif, France.

出版信息

Cell Biol Int Rep. 1988 Feb;12(2):115-22. doi: 10.1016/0309-1651(88)90125-7.

DOI:10.1016/0309-1651(88)90125-7

PMID:3396078

Abstract

The rate of attachment of chick embryo fibroblasts (CEF) incubated for 1 h at pH 6 in the absence of serum, was equal to about 90% with 2% of spreading cells. The addition of 1% horse serum (HS) decreased to about 30% the rate of attached cells but increased to about 30% the proportion of spreading cells. We found that 3 microM cobalt-protoporphyrin IX (CoPP) added for 3 min before the addition of 1% HS increased both the rate of cell attachment (about 60%) and the proportion of cell spreading (about 50%).

摘要

在无血清条件下于pH 6孵育1小时的鸡胚成纤维细胞（CEF）的附着率，约90%的细胞附着，其中2%为铺展细胞。添加1%马血清（HS）后，附着细胞率降至约30%，但铺展细胞比例增至约30%。我们发现，在添加1% HS前3分钟添加3 microM钴原卟啉IX（CoPP），可使细胞附着率（约60%）和细胞铺展比例（约50%）均增加。