Bhat P V, Co H T, Lacroix A
J Chromatogr. 1983 Apr 29;260(1):129-36. doi: 10.1016/0021-9673(83)80015-6.
The effects of 2-alkanols as mobile phase modifiers on the resolution of 11-cis-, 13-cis-, 9-cis- and all-trans-retinol in high-performance liquid chromatography were studied. Partisil-10-ODS and Zorbax CN columns were used in series. The retentions of 9-cis-and all-trans-retinol were significantly influenced by the presence of long-chain 2-alkanols in the mobile phase. Baseline separation of 9-cis-and all-trans-retinol was achieved when 1% 2-heptanol in hexane was used as the eluent. On the other hand, the separation of 11-cis-from 13-cis-retinol required 5% dioxane in hexane. The method is applicable to the separation of retinol isomers present in biological samples. Analysis of normal rat liver lipids showed the presence of a small amount of 13-cis-retinol in addition to all-trans-retinol.
研究了2-链烷醇作为流动相改性剂对高效液相色谱中11-顺式、13-顺式、9-顺式和全反式视黄醇分离度的影响。串联使用Partisil-10-ODS柱和Zorbax CN柱。流动相中长链2-链烷醇的存在对9-顺式和全反式视黄醇的保留有显著影响。当使用己烷中1%的2-庚醇作为洗脱剂时，实现了9-顺式和全反式视黄醇的基线分离。另一方面，11-顺式视黄醇与13-顺式视黄醇的分离需要己烷中5%的二氧六环。该方法适用于生物样品中视黄醇异构体的分离。对正常大鼠肝脏脂质的分析表明，除全反式视黄醇外，还存在少量的13-顺式视黄醇。
