Buescher E S, Gallin J I
J Immunol Methods. 1983 Jul 15;61(2):141-4. doi: 10.1016/0022-1759(83)90155-2.
Conventional methods for leukocyte rosetting suffer from 2 major drawbacks: the length of time required for leukocyte purification prior to rosetting, and the necessity for large volumes of blood from which to purify the leukocytes. A simple method for leukocyte rosetting is described which uses small quantities (less than 100 microliter) of heparinized whole blood, does not require prior leukocyte purification and can be completed within 1 h of blood drawing. Percentages of rosetting polymorphonuclear leukocytes in cell preparations obtained by standard purification methods showed a close correlation (P less than 0.001) with percentages obtained by the method described. Additionally, the method described can potentially be applied to other non-neutrophil rosetting leukocytes in blood or body fluids.
玫瑰花结形成前白细胞纯化所需的时间，以及从大量血液中纯化白细胞的必要性。本文描述了一种简单的白细胞玫瑰花结形成方法，该方法使用少量（少于100微升）肝素化全血，无需事先纯化白细胞，并且可以在采血后1小时内完成。通过标准纯化方法获得的细胞制剂中玫瑰花结形成的多形核白细胞百分比与所述方法获得的百分比显示出密切相关性（P小于0.001）。此外，所述方法可能适用于血液或体液中的其他非中性粒细胞玫瑰花结形成白细胞。
