内皮细胞生长补充物：一种促进产生单克隆抗体的杂交瘤细胞生长的细胞克隆因子。

Endothelial cell growth supplement: a cell cloning factor that promotes the growth of monoclonal antibody producing hybridoma cells.

作者信息

Pintus C, Ransom J H, Evans C H

出版信息

J Immunol Methods. 1983 Jul 15;61(2):195-200. doi: 10.1016/0022-1759(83)90162-x.

Abstract

The efficiency of endothelial cell growth supplement (ECGS), a commercially marketed extract of bovine neural tissue, human endothelial cell supernatant (HECS) derived from freshly isolated endothelial cells, and feeder layers of murine peritoneal cells (PEC), were compared for their ability to support cell fusion, clonal growth, and monoclonal antibody production of murine hybridoma cells. ECGS at 25-100 micrograms/ml was similar to a 1:5 dilution of HECS in supporting the growth of hybridoma colonies; both ECGS and HECS were superior to PEC feeder cells. Furthermore, hybridomas cloned in ECGS produced anti-lymphotoxin antibodies. The commercial availability and stability of ECGS together with its ability anti-lymphotoxin antibodies. The commercial availability and stability of ECGS a superior growth supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

摘要

对市售的牛神经组织提取物内皮细胞生长补充剂（ECGS）、源自新鲜分离内皮细胞的人内皮细胞上清液（HECS）以及小鼠腹膜细胞饲养层（PEC）在支持小鼠杂交瘤细胞的细胞融合、克隆生长和单克隆抗体产生方面的能力进行了比较。25至100微克/毫升的ECGS在支持杂交瘤集落生长方面与1:5稀释的HECS相似；ECGS和HECS均优于PEC饲养细胞。此外，在ECGS中克隆的杂交瘤产生了抗淋巴毒素抗体。ECGS的商业可用性和稳定性及其产生抗淋巴毒素抗体的能力。ECGS的商业可用性和稳定性使其成为单克隆抗体生产中杂交瘤细胞融合和生长的优质生长补充剂。