Human endothelial culture supernatant selectively promotes IgM-producing hybridomas.
作者信息
Greferath R, Sauer-Nehls S, Dyballa S, Nehls P
机构信息
Center for Blood Research Laboratories, Harvard Medical School, Boston, MA 02135, USA.
出版信息
J Immunol Methods. 1997 May 26;204(2):189-92. doi: 10.1016/s0022-1759(97)00046-x.
PMID:9212836
Abstract
We compared the effect of human endothelial cell culture supernatant (HECS), ESG (Ewing sarcoma growth factor), IL-6 (interleukin-6) and peritoneal macrophages on the recovery of hybridoma cells after fusion with respect to growth, stability and distribution of isotype variants. A selective growth of murine IgM-producing hybridoma cells was observed in the presence of HECS after cell fusion.
摘要
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