Kubilus J
J Invest Dermatol. 1983 Jul;81(1 Suppl):55s-8s. doi: 10.1111/1523-1747.ep12540570.
At high levels of vitamin A and other retinoids (3 X 10(-6) M) attachment of human keratinocytes to 3T3-coated plastic dishes is mildly inhibited. Retinoids at this concentration in culture media seem to have an antikeratinizing effect in that cells appear to be less differentiated. Retinoid-treated cultures are less stratified, having fewer cell layers, and display larger intercellular spaces and rounder, less flattened cells. Treated cultures also contain higher percentages of saline-soluble proteins and lower percentages of proteins requiring reduction and/or denaturants for solubility. This suggests that in treated cultures, the most keratinized cells are absent. Growth curves show that those most keratinized cells are sloughed from the dish and appear in the media. Thus at 3 X 10(-6) M, the major retinoid effect is to promote desquamation. At higher concentrations, retinoids are toxic to the keratinocyte, but at lower concentrations, they may be stimulative.
在高浓度维生素A及其他类视黄醇（3×10⁻⁶ M）的作用下，人角质形成细胞与3T3包被的塑料培养皿的附着受到轻度抑制。培养基中该浓度的类视黄醇似乎具有抗角质化作用，因为细胞看起来分化程度较低。经类视黄醇处理的培养物分层较少，细胞层数较少，细胞间间隙较大，细胞更圆且扁平化程度较低。经处理的培养物中盐溶性蛋白质的百分比也更高，而需要还原和/或变性剂才能溶解的蛋白质百分比更低。这表明在经处理的培养物中，最角质化的细胞不存在。生长曲线显示，那些角质化程度最高的细胞从培养皿上脱落并出现在培养基中。因此，在3×10⁻⁶ M时，类视黄醇的主要作用是促进脱屑。在更高浓度下，类视黄醇对角质形成细胞有毒性，但在较低浓度下，它们可能具有刺激作用。
