Seeley P J, Keith C H, Shelanski M L, Greene L A
J Neurosci. 1983 Jul;3(7):1488-94. doi: 10.1523/JNEUROSCI.03-07-01488.1983.
Exogenous proteins have been introduced into naive and nerve growth factor (NGF)-primed pheochromocytoma cells by pressure microinjection. Microinjection of cells did affect NGF-induced neurite outgrowth by these cells over periods of up to 3 days. Injected NGF failed to elicit neurite outgrowth or regeneration in the absence of extracellular NGF and failed to enhance it in its presence. Injected anti-NGF IgG failed to inhibit neurite outgrowth or regeneration induced by extracellular NGF. Similar results were obtained for both nuclear and cytoplasmic injections.
通过压力显微注射已将外源性蛋白质导入未致敏的和经神经生长因子（NGF）预处理的嗜铬细胞瘤细胞中。在长达3天的时间内，对细胞进行显微注射确实影响了这些细胞由NGF诱导的神经突生长。在没有细胞外NGF的情况下，注射的NGF未能引发神经突生长或再生，而在有细胞外NGF存在时也未能增强其作用。注射的抗NGF IgG未能抑制由细胞外NGF诱导的神经突生长或再生。核内注射和胞质注射均获得了相似的结果。
