Bickhardt K, Büttner D, Müschen U, Plonait H
Lab Anim. 1983 Apr;17(2):161-5. doi: 10.1258/002367783780959565.
The influence of experimental conditions on 10 haematological and blood chemical constituents were investigated in male Han:Wistar rats. Significant differences existed between values of haematological and chemical constituents of blood, sampled within 10 s, between 10 and 30, or between 30 and 60 s after decapitation respectively, The leucocyte count decreased quickly but the lactate concentration rose after decapitation. The interindividual coefficient of variance for the majority of the blood constituents was reduced markedly if blood was sampled within 10 s of decapitation. Significant differences in plasma protein and blood lactate concentrations existed between 2 rats kept in one cage, when the first rat was decapitated 20 s before the second one. Plasma protein concentrations differed significantly between rats bled at 9.00 am and 11.00 am. Significant differences in blood glucose and lactate concentrations existed between rats bled on 5 different days within a period of 10 days. Packed cell volume, eosinophilic granulocytes and plasma protein concentration differed significantly between rats kept in a room with a 12:12 light: darkness (LD) cycle and those living in a room with a 2.3:2.3 LD cycle.
在雄性Han:Wistar大鼠中研究了实验条件对10种血液学和血液化学成分的影响。分别在断头后10秒内、10至30秒或30至60秒采集的血液，其血液学和化学成分的值之间存在显著差异。断头后白细胞计数迅速下降，但乳酸浓度升高。如果在断头后10秒内采集血液，大多数血液成分的个体间变异系数会显著降低。当第一只大鼠在第二只大鼠断头前20秒断头时，饲养在一个笼子里的两只大鼠之间的血浆蛋白和血液乳酸浓度存在显著差异。上午9点和11点采血的大鼠之间血浆蛋白浓度有显著差异。在10天内的5个不同日子采血的大鼠之间，血糖和乳酸浓度存在显著差异。饲养在12:12光照:黑暗（LD）周期房间的大鼠与生活在2.3:2.3 LD周期房间的大鼠之间，红细胞压积、嗜酸性粒细胞和血浆蛋白浓度有显著差异。
