Hendriks F F, Jonas J, van der Laarse A, Huysmans H A
J Surg Res. 1983 Jul;35(1):41-9. doi: 10.1016/0022-4804(83)90124-5.
An isolated working rat heart model designed for function studies after elective cardiac arrest is described. Before and after a cold ischemic period (induced with St. Thomas' cardioplegic solution) of 30 min, hemodynamic and metabolic variables were assessed in a group of 13 hearts perfused with a modified Krebs-Henseleit solution and in a group of 13 hearts perfused with reconstituted blood. Functional recovery was about 90% in the nonblood-perfusate group and 98% in the blood-perfused group. Functional deterioration in time due to edema formation and limited oxygenation in electrolyte-perfused hearts was found to be of the same magnitude as the function loss ascribed to deleterious effects of cardioplegic arrest. The blood-perfused hearts showed hemodynamic stability during a 75 min continuous working period, and complete recovery of function after 30 min arrest.
本文描述了一种用于选择性心脏骤停后功能研究的离体工作大鼠心脏模型。在30分钟的冷缺血期（用圣托马斯心脏停搏液诱导）前后，对一组用改良克雷布斯 - 亨塞尔特溶液灌注的13颗心脏和一组用重组血液灌注的13颗心脏的血流动力学和代谢变量进行了评估。非血液灌注组的功能恢复约为90%，血液灌注组为98%。发现电解质灌注心脏中由于水肿形成和氧合受限导致的功能随时间恶化的程度与心脏停搏有害作用导致的功能丧失程度相同。血液灌注的心脏在75分钟的连续工作期内表现出血流动力学稳定性，在30分钟停搏后功能完全恢复。
