Karlaganis G, Bremmelgaard A, Karlaganis V, Sjövall J
J Steroid Biochem. 1983 Jun;18(6):725-9. doi: 10.1016/0022-4731(83)90251-0.
Cholesterol was shown to be the precursor of 27-nor-5 beta-cholestane-3 alpha, 7 alpha, 12 alpha, 24,25-pentol which is the major bile alcohol in human urine. 4-[14C]-Labelled cholesterol and beta-sitosterol were administered to patients with primary biliary cirrhosis. Urine was extracted with Amberlite XAD-2 and sterol glucuronides and bile acid conjugates were isolated by ion exchange chromatography on Lipidex-DEAP. Following hydrolysis and further purification on Lipidex-DEAP, the C26 bile alcohol and methyl esters of cholic and chenodeoxycholic acids were isolated by HPLC. The specific radioactivity of the C26-pentol was the same as that of cholic acid after administration of [14C]-cholesterol. In contrast, little if any radioactivity could be detected in the C26-pentol after administration of labelled beta-sitosterol.
胆固醇被证明是27-降-5β-胆甾烷-3α，7α，12α，24,25-戊醇的前体，该物质是人类尿液中的主要胆汁醇。将4-[¹⁴C]标记的胆固醇和β-谷甾醇给予原发性胆汁性肝硬化患者。尿液用Amberlite XAD - 2萃取，甾醇葡糖醛酸苷和胆汁酸结合物通过Lipidex - DEAP离子交换色谱法分离。水解并在Lipidex - DEAP上进一步纯化后，通过高效液相色谱法分离出C26胆汁醇以及胆酸和鹅去氧胆酸的甲酯。给予[¹⁴C] - 胆固醇后，C26 - 戊醇的比放射性与胆酸相同。相比之下，给予标记的β-谷甾醇后，在C26 - 戊醇中几乎检测不到放射性。
