Katsumata Y, Sato K, Yada S, Suzuki O, Yoshino M
Life Sci. 1983 Jul 11;33(2):151-5. doi: 10.1016/0024-3205(83)90407-1.
In order to investigate the changes in energy metabolism during acute anoxia, blood levels of various metabolites were analysed in cyanide-poisoned rats. After intraperitoneal injection of a sublethal dose of potassium cyanide (5 mg/kg), blood samples were obtained by cervical dislocation at intervals of 5 min until 30 min. Lactate and lactate/pyruvate ratio (L/P) in plasma concomitantly changed with cyanide; increased rapidly at 5 min, remained fairly constant until 20 min and then began to decrease at 25 min. In contrast, the products of ATP degradation, oxypurines and inorganic phosphate (Pi), increased gradually until 25 min and then began to decrease at 30 min. Allantoin in plasma scarcely increased throughout the experiments. The results indicate that the rapid activation of anaerobic ATP formation by glycolysis was followed by the increase in ATP degradation in cyanide-poisoned rats, Thus, increase in plasma oxypurines could be regarded as an indicator for severe anoxic states in tissues with massive ATP degradation.
为了研究急性缺氧期间能量代谢的变化，对氰化物中毒的大鼠分析了各种代谢物的血液水平。腹腔注射亚致死剂量的氰化钾（5毫克/千克）后，每隔5分钟通过颈椎脱臼采集血样，直至30分钟。血浆中的乳酸和乳酸/丙酮酸比值（L/P）随氰化物而同时变化；在5分钟时迅速增加，直到20分钟保持相当稳定，然后在25分钟时开始下降。相反，ATP降解产物、氧嘌呤和无机磷酸盐（Pi）在25分钟前逐渐增加，然后在30分钟时开始下降。在整个实验过程中，血浆中的尿囊素几乎没有增加。结果表明，在氰化物中毒的大鼠中，糖酵解迅速激活无氧ATP生成，随后ATP降解增加。因此，血浆氧嘌呤的增加可被视为大量ATP降解的组织中严重缺氧状态的指标。
