Tsutsumi H, Sato K, Tamaki K, Mizuno Y, Katsumata Y
Criminal Science Laboratory, Aichi Prefectural Police Headquarters, Nagoya, Japan.
Int J Legal Med. 1991 Mar;104(2):105-8. doi: 10.1007/BF01626040.
Experimental stains of human urine prepared on 4 different types of cloth and cloth stains in practical cases were examined by 2 methods which have been established in this laboratory. A chemical method determining uric acid and urea nitrogen content and an enzyme-linked immunosorbent assay (ELISA) to determine uromucoid. The former method uses the quotient uric acid/urea nitrogen as an indicator and the latter the quotient ELISA score/protein concentration. All stains were identified as human urine stains by both methods when the specimens were taken from the centre of the stains but when taken from the periphery some of them, especially those on cloth such as cotton or silk, could not be identified as human urine stains. These results indicate that the central part of stains should be preferentially used for the identification of human urine stains, especially those made on cloth such as cotton or silk.
采用本实验室建立的两种方法，对在4种不同类型布料上制备的人尿实验污渍以及实际案例中的布料污渍进行了检测。一种是测定尿酸和尿素氮含量的化学方法，另一种是测定尿类粘蛋白的酶联免疫吸附测定法（ELISA）。前一种方法使用尿酸/尿素氮的比值作为指标，后一种方法使用ELISA评分/蛋白质浓度的比值。当从污渍中心取样时，两种方法都能将所有污渍鉴定为人尿污渍，但当从污渍边缘取样时，其中一些污渍，尤其是那些在棉布或丝绸等布料上的污渍，无法被鉴定为人尿污渍。这些结果表明，污渍的中心部分应优先用于人尿污渍的鉴定，尤其是那些在棉布或丝绸等布料上形成的污渍。
