大鼠心脏的体内乙酰胆碱周转率
In vivo acetylcholine turnover in rat heart.
作者信息
Brown O M, Salata J J
出版信息
Life Sci. 1983 Jul 18;33(3):213-24. doi: 10.1016/0024-3205(83)90379-x.
The in vivo uptake of choline (Ch) and synthesis of acetylcholine (ACh) in rat heart were studied using a pyrolysis mass fragmentography (PMF) method. Deuterium labeled Ch was pulse injected (i.v.) into anesthetized rats. Labeled and unlabeled Ch and ACh were measured by PMF in hearts at various times following injection. From these data we calculated the specific activities of Ch and ACh, rate constants for ACh and turnover rates of ACh. After an initial equilibration period of approximately 2 min, the specific activities of Ch and ACh decayed in a parallel manner with half-times of 28.2 and 28.8 min respectively. Between 2 and 60 min the calculated ACh turnover rate was 0.144 nmol/g/min. Unlike brain Ch, heart Ch levels are very stable with time following sacrifice. No advantage was found in using microwave irradiation to stabilize heart ACh and Ch content.
采用热解质谱碎片分析法（PMF）研究了大鼠心脏中胆碱（Ch）的体内摄取及乙酰胆碱（ACh）的合成。将氘标记的Ch脉冲静脉注射到麻醉大鼠体内。注射后不同时间通过PMF测量心脏中标记和未标记的Ch及ACh。根据这些数据，我们计算了Ch和ACh的比活性、ACh的速率常数及ACh的周转率。在约2分钟的初始平衡期后，Ch和ACh的比活性以平行方式衰减，半衰期分别为28.2分钟和28.8分钟。在2至60分钟之间，计算出的ACh周转率为0.144 nmol/g/分钟。与脑Ch不同，心脏Ch水平在处死动物后随时间非常稳定。未发现使用微波辐射来稳定心脏ACh和Ch含量有任何优势。
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