Grosovsky A J, Little J B
Mutat Res. 1983 Jun-Jul;110(1):163-70. doi: 10.1016/0027-5107(83)90025-8.
The influence of growth rate on the kinetics of phenotypic expression of 6-thioguanine resistance has been investigated by establishing 3 protocols for the maintenance of mutagenized stock cultures of human diploid fibroblasts. These protocols differed in the frequency with which subculture was performed during the phenotypic expression period. The resultant growth kinetics varied widely from continual exponential proliferation to density-inhibited growth for much of the expression period. With all of the subculture protocols, a maximum mutation frequency was reached by 9 days after exposure of confluent cultures to 6 J/m2 of 254 nm UV light. The maximum frequency was similar in all experimental groups and remained stable up to 17 days after irradiation, the last time point tested. These results indicate that continual proliferation is not required for optimal expression of 6-thioguanine resistance.
通过建立3种维持人二倍体成纤维细胞诱变原种培养物的方案，研究了生长速率对6-硫鸟嘌呤抗性表型表达动力学的影响。这些方案在表型表达期传代培养的频率上有所不同。在大部分表达期内，由此产生的生长动力学差异很大，从持续的指数增殖到密度抑制生长。对于所有传代培养方案，汇合培养物暴露于6 J/m2的254 nm紫外线后9天达到最大突变频率。所有实验组的最大频率相似，并且在辐照后长达17天（测试的最后一个时间点）保持稳定。这些结果表明，6-硫鸟嘌呤抗性的最佳表达不需要持续增殖。
