Simpson E L, Mundy G R, D'Souza S M, Ibbotson K J, Bockman R, Jacobs J W
N Engl J Med. 1983 Aug 11;309(6):325-30. doi: 10.1056/NEJM198308113090601.
We used a sensitive and specific hybridization assay that detects evidence of parathyroid hormone synthesis in tumors to investigate whether this hormone mediates the hypercalcemia of malignant disease. The assay uses radiolabeled, cloned parathyroid hormone DNA to hybridize selectively with parathyroid hormone messenger RNA. We assayed 13 human and 3 animal tumors of diverse cell origins that are frequently associated with the hypercalcemia of cancer. Five of the human tumors were obtained from patients known to be hypercalcemic at the time of tumor excision, two were from normocalcemic patients, and six were from patients with breast cancer whose serum calcium levels were unknown. Messenger RNA was prepared from cultured cell lines or tumors; active RNA fractions were hybridized with either human or bovine cloned parathyroid hormone DNA that had been labeled to a high specific activity with [32P]nucleotide. We were unable to detect parathyroid hormone RNA transcripts in any of the tumors. Our results indicate that parathyroid hormone rarely, if ever, causes hypercalcemia in malignant disease.
我们使用了一种灵敏且特异的杂交检测法来研究甲状旁腺激素是否介导恶性疾病中的高钙血症，该检测法可检测肿瘤中甲状旁腺激素合成的证据。该检测法使用放射性标记的克隆甲状旁腺激素DNA与甲状旁腺激素信使RNA进行选择性杂交。我们检测了13例人类肿瘤和3例动物肿瘤，这些肿瘤来源于不同的细胞，且常伴有癌症高钙血症。其中5例人类肿瘤取自肿瘤切除时已知高钙血症的患者，2例取自血钙正常的患者，6例取自血清钙水平未知的乳腺癌患者。信使RNA是从培养的细胞系或肿瘤中制备的；活性RNA组分与用人或牛克隆甲状旁腺激素DNA杂交，该DNA已用[32P]核苷酸标记至高比活性。我们在任何肿瘤中均未检测到甲状旁腺激素RNA转录本。我们的结果表明，甲状旁腺激素在恶性疾病中极少（如果有的话）引起高钙血症。
