三磷酸核苷木糖A2'-5'木糖A2'-5'木糖的构象特征。核磁共振和圆二色性研究。
Conformational characteristics of the trinucleoside diphosphate xyloA2'-5'xyloA2'-5'xyloA. A nuclear magnetic resonance and CD study.
作者信息
Doornbos J, Gosselin G, Imbach J L, Altona C
出版信息
Nucleic Acids Res. 1983 Jul 11;11(13):4553-67. doi: 10.1093/nar/11.13.4553.
Abstract
In this paper the conformational analysis of the 2'-5' linked xylotrinucleotide xA2'-5'xA2'-5'xA is reported. The title compound is an analogue of A2'-5'A2'-5'A, which compound was shown to display inhibitive effects on protein synthesis. The complete 1H-NMR assignment of the high field spectral region of the xylose trimer is given. Modes of base-base stacking are extracted from coupling constant data at various temperatures. Circular dichroic (CD) spectra confirm the presence of stacked states at low temperature. Xylonucleosides are known to prefer the N-type sugar conformation. However, in the present trimer the S-type conformer is suggested to partake in stacked conformations. Two types of stacking in the two constituent dimer fragments of the trimer are proposed to rationalize the NMR data: xA(1)N-xA(2)S and xA(2)N-xA(3)S.
摘要
本文报道了2'-5'连接的木糖三核苷酸xA2'-5'xA2'-5'xA的构象分析。标题化合物是A2'-5'A2'-5'A的类似物，已表明该化合物对蛋白质合成具有抑制作用。给出了木糖三聚体高场光谱区域的完整1H-NMR归属。从不同温度下的耦合常数数据中提取碱基-碱基堆积模式。圆二色（CD）光谱证实了低温下存在堆积态。已知木糖核苷偏好N型糖构象。然而，在当前的三聚体中，S型构象异构体被认为参与了堆积构象。为合理解释NMR数据，提出了三聚体两个组成二聚体片段中的两种堆积类型：xA(1)N-xA(2)S和xA(2)N-xA(3)S。
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Conformational analysis of the nucleotides A2'-5'A, A2'-5'A2'-5'A and A2'-5'U from nuclear magnetic resonance and circular dichroism studies.通过核磁共振和圆二色性研究对核苷酸A2'-5'A、A2'-5'A2'-5'A和A2'-5'U进行构象分析。
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Thermodynamics of stacking and of self-association of the dinucleoside monophosphate m2(6)A-U from proton NMR chemical shifts: differential concentration temperature profile method.基于质子核磁共振化学位移的二磷酸单核苷m2(6)A-U堆积与自缔合的热力学：差分浓度-温度分布法
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