三磷酸二核苷3'd(A2'-5'A2'-5'A)的构象分析。一项核磁共振和圆二色性研究。
Conformational analysis of the trinucleoside diphosphate 3'd(A2'-5'A2'-5'A). An NMR and CD study.
作者信息
Doornbos J, Charubala R, Pfleiderer W, Altona C
出版信息
Nucleic Acids Res. 1983 Jul 11;11(13):4569-82. doi: 10.1093/nar/11.13.4569.
Abstract
A 500 MHz and 300 MHz NMR study of the trinucleoside diphosphate 3'd(A2'-5'A2'-5'A) is presented. In addition, circular dichroism is used to study base stacking in the title compound. The complete 1H-NMR spectral assignment of the sugar ring proton signals is given. Information about the sugar ring (N- or S-type conformation) and about the backbone geometry along C4'-C5' and C5'-O5' bonds is obtained from the NMR coupling constants. It is shown that the trimer mainly occurs in the N-N-N stacked state at low temperatures; the presence of a minor amount of N-N-S conformational sequence is indicated.
摘要
本文介绍了对三磷酸二核苷3'd(A2'-5'A2'-5'A)进行的500兆赫兹和300兆赫兹核磁共振研究。此外，还利用圆二色性研究了标题化合物中的碱基堆积。给出了糖环质子信号的完整1H-核磁共振光谱归属。从核磁共振耦合常数获得了有关糖环（N型或S型构象）以及沿C4'-C5'和C5'-O5'键的主链几何结构的信息。结果表明，三聚体在低温下主要以N-N-N堆积状态存在；表明存在少量的N-N-S构象序列。
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