Fujita K, Yamada Y, Azuma K, Hirozawa S
Antimicrob Agents Chemother. 1978 Jul;14(1):132-6. doi: 10.1128/AAC.14.1.132.
The antifungal activity of a lyophilized powder containing aloe leaf homogenate (whole-leaf powder) against Trichophyton mentagrophytes was investigated. The minimal inhibitory concentration was 25 mg/ml by the agar dilution method, using Sabouraud glucose agar medium. At subinhibitory concentrations, the powder exerted its main effect on colony growth by prolongation of the lag phase and inhibition of growth rate. Homogenates of fresh whole leaf were filtered through Whatman GF/A paper, and the filtrate was dialyzed and concentrated by molecular filtration using an Amicon hollow-fiber dialyzer concentrator DC-2, and a powder containing components with molecular weights higher than 10,000 (high-molecular-weight component powder) was prepared by lyophilization. The minimal inhibitory concentrations against three strains of T. mentagrophytes were all 10 mg/ml. The inhibitory activity was fungicidal and was lost by heating at 100 degrees C for 30 min. Both the whole-leaf powder and the high-molecular-weight component powder induced various morphological abnormalities in spores and hyphae by the inhibition of spore germination and development of hyphae.
研究了含有芦荟叶匀浆的冻干粉末（全叶粉末）对须癣毛癣菌的抗真菌活性。采用琼脂稀释法，使用沙氏葡萄糖琼脂培养基，其最低抑菌浓度为25mg/ml。在亚抑菌浓度下，该粉末主要通过延长迟缓期和抑制生长速率来影响菌落生长。将新鲜全叶匀浆通过Whatman GF/A滤纸过滤，滤液经透析并用Amicon中空纤维透析浓缩器DC-2进行分子过滤浓缩，冻干后制备得到含有分子量高于10000的成分的粉末（高分子量成分粉末）。对三株须癣毛癣菌的最低抑菌浓度均为10mg/ml。其抑制活性具有杀菌作用，在100℃加热30分钟后活性丧失。全叶粉末和高分子量成分粉末均通过抑制孢子萌发和菌丝发育，诱导孢子和菌丝出现各种形态异常。
