2-脱氧链霉胺的定性测定方法
Method for qualitative determination of 2-deoxystreptamine.
作者信息
Lee B K, Condon R G, Patel M, Oden E M, Waitz J A
出版信息
Antimicrob Agents Chemother. 1978 Jul;14(1):157-8. doi: 10.1128/AAC.14.1.157.
Abstract
We have developed a qualitative method to assay for the presence of 2-deoxystreptamine in hydrolysates of crude aminoglycoside preparations using a 2-deoxystreptamine-requiring idiotrophic mutant. The assay involves (i) incubation of a 2-deoxystreptamine-requiring mutant with 1 mg of a hydrolyzed preparation from a crude unknown antibiotic mixture per ml, and (ii) examination of the resultant incubation mixture for production of antibiotic(s) by disk assay.
摘要
我们开发了一种定性方法，使用一种需要2-脱氧链霉胺的营养缺陷型突变体来检测粗制氨基糖苷制剂水解产物中2-脱氧链霉胺的存在。该检测方法包括：(i) 将每毫升含有1毫克来自粗制未知抗生素混合物的水解制剂与需要2-脱氧链霉胺的突变体一起孵育；(ii) 通过纸片法检测所得孵育混合物中抗生素的产生情况。
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