Golub M S, Gershwin M E, Vijayan V K
Physiol Behav. 1983 Mar;30(3):409-13. doi: 10.1016/0031-9384(83)90145-2.
Swiss-Webster outbred mice were fed marginally or severely zinc deficient diets (9 or 2 ppm zinc) from day 16 gestation to day 15 postnatal. Control mice were fed a 100 ppm diet either ad lib or in amounts equal to the diet intake of deprived mice (pair fed controls). Male and female offspring were tested at 70 days of age in a one-trial passive avoidance task with a 30 min train-test interval. Both marginally and severely zinc deprived offspring (but not pair fed controls) had shorter avoidance latencies than offspring of ad lib fed zinc replete controls. Zinc deprived offspring did not differ from control mice when either baseline or "stressed" (exposure to novel environment) plasma corticosterone levels were quantitated. Further, zinc staining patterns of the hippocampus (Timm-sulfide stain) were not altered in the nutritionally deprived offspring. Thus marginal dietary zinc deficiency during development can lead to impaired passive avoidance performance in adult mice. This behavioral effect is not associated with altered pituitary-adrenocortical activity or with a permanent reduction in hippocampal zinc staining. This result has significant implications for the influence of zinc deprivation in utero and in the neonatal animal on adult behavior characteristics.
从妊娠第16天到出生后第15天，给瑞士韦伯斯特远交系小鼠喂食轻度或重度缺锌饮食（锌含量分别为9 ppm或2 ppm）。对照小鼠自由采食100 ppm的饮食，或者采食与缺锌小鼠饮食摄入量相同的量（配对喂食对照）。在一项单次被动回避任务中，对70日龄的雄性和雌性后代进行测试，训练-测试间隔为30分钟。轻度和重度缺锌的后代（但不包括配对喂食对照）的回避潜伏期比自由采食锌充足对照的后代短。当对基线或“应激”（暴露于新环境）血浆皮质酮水平进行定量时，缺锌后代与对照小鼠没有差异。此外，营养缺乏后代海马体的锌染色模式（硫代硫酸亚铊染色）没有改变。因此，发育期间轻度饮食缺锌会导致成年小鼠被动回避行为受损。这种行为效应与垂体-肾上腺皮质活动改变或海马体锌染色的永久性减少无关。这一结果对于子宫内和新生动物缺锌对成年行为特征的影响具有重要意义。
