Rehfeld J F
Regul Pept. 1983 Apr;6(1):33-41. doi: 10.1016/0167-0115(83)90132-5.
Dibutyryl cyclic GMP has been reported to interact with antisera specific for C-terminal tetrapeptide amide common for cholecystokinin (CCK) and gastrin. Moreover, cyclic nucleotides elute by gel chromatography in the same position as the free CCK/gastrin tetrapeptide. Therefore, we have examined the reactivity of 25 mononucleotides with eight CCK and gastrin antisera. The results show that the nucleotides all bind poorly to the antisera (nucleotide concentration required greater than 1 mM). Hence, endogenous cyclic nucleotides, which are present in biological extracts in pM to nM concentrations, do not interfere with immunochemical CCK or gastrin measurements. The antisera displayed highly individual patterns of reactivity without preferential binding of di- or monobutyryl cyclic nucleotides (AMP, GMP or IMP). Thus, the present results do not support the idea of structural resemblance between the C-terminus of CCK/gastrin peptides and butyryl derivatives of cyclic GMP. Enzymatic treatment of the antral tetrapeptide-like immunoreactivity showed that nucleotides do not contribute to this material, which appears exclusively peptidergic.
据报道，二丁酰环鸟苷酸可与针对胆囊收缩素（CCK）和胃泌素共有的C末端四肽酰胺的抗血清发生相互作用。此外，环核苷酸在凝胶色谱中的洗脱位置与游离CCK/胃泌素四肽相同。因此，我们检测了25种单核苷酸与8种CCK和胃泌素抗血清的反应性。结果表明，核苷酸与抗血清的结合都很差（所需核苷酸浓度大于1 mM）。因此，生物提取物中以皮摩尔至纳摩尔浓度存在的内源性环核苷酸不会干扰免疫化学法检测CCK或胃泌素。抗血清表现出高度个体化的反应模式，对二丁酰或单丁酰环核苷酸（AMP、GMP或IMP）没有优先结合。因此，目前的结果不支持CCK/胃泌素肽的C末端与环鸟苷酸的丁酰衍生物之间存在结构相似性的观点。对胃窦四肽样免疫反应性的酶处理表明，核苷酸对这种物质没有贡献，这种物质似乎完全是肽能性的。
