一种促肾因子的部分特性分析。

Partial characterization of a renotropic factor.

作者信息

Gaydos D S, Goldin H, Jenson B, Gersten D, Boedeker B, Bartz C, Preuss H G

出版信息

Ren Physiol. 1983;6(3):139-44. doi: 10.1159/000172892.

Abstract

In 70 experiments, the existence of a circulating renal growth factor was confirmed by 9.3% stimulation of 3H-thymidine into the DNA of renal fragments incubating for 90 min in the presence of sera from 20-hour unilaterally nephrectomized rats compared to sera from 20-hour sham-operated rats (p less than 0.001). Dialysis (7.4%, p less than 0.01) or removal of albumin (11.8%, p less than 0.001) from sera of both sham-operated and unilaterally nephrectomized rats did not appreciably change the magnitude of the statistically significant stimulation. When albumin-free sera were placed in boiling water for 1-3 min to coagulate protein, the stimulation was still significantly different from control (5%, p less than 0.05). Addition of sera from unilaterally nephrectomized rats (20 h) to isolated nuclei, even isolated nuclei removed from growing kidneys (72 h after unilateral nephroctomy), failed to enhance DNA synthesis significantly.

摘要

在70项实验中，与假手术20小时大鼠的血清相比，单侧肾切除20小时大鼠的血清存在循环肾生长因子，这通过在其存在下将3H-胸腺嘧啶掺入孵育90分钟的肾片段DNA中产生9.3%的刺激得以证实（p<0.001）。对假手术和单侧肾切除大鼠的血清进行透析（7.4%，p<0.01）或去除白蛋白（11.8%，p<0.001），并未明显改变具有统计学意义的刺激程度。当将无白蛋白血清置于沸水中1至3分钟使蛋白质凝固时，刺激仍与对照有显著差异（5%，p<0.05）。将单侧肾切除20小时大鼠的血清添加到分离的细胞核中，即使是从生长中的肾脏（单侧肾切除72小时后）分离出的细胞核，也未能显著增强DNA合成。