Preuss H G, Goldin H, Shivers M
Yale J Biol Med. 1978 May-Jun;51(3):403-12.
Based upon many investigations, the existence of short-lived, specific, circulating substances which incite and/or regulate compensatory renal growth has been proposed. In our studies, we find that sera and plasma from unilaterally nephrectomized rats compared to sera and plasma from sham-operated rats stimulate the incorporation of (3)H-thymidine monophosphate, (3)H-thymidine and (14)C-uridine into the DNA of incubating rat kidney fragments. While extracts from growing rat kidneys are not excitatory, they produce a relative enhancement to incorporation of isotope into DNA when combined with sera from uninephrectomized rats-more than the sera do alone. The above is found also for the incorporation of (14)C-uridine into RNA of incubating rat kidney fragments. Sera from uninephrectomized rats fail to stimulate DNA synthesis in liver slices from rats but do so in the presence of extracts from growing kidneys. Renotropic factors in sera and extracts do not appear to work by diluting the isotopes, by enhancing transport, or by effecting overall metabolism of the renal cells. The above described serum and liver factors may play a role in compensatory renal growth.
基于多项研究，有人提出存在能刺激和/或调节肾脏代偿性生长的短暂存在的特定循环物质。在我们的研究中，我们发现，与假手术大鼠的血清和血浆相比，单侧肾切除大鼠的血清和血浆能刺激将（3）H - 胸腺嘧啶单磷酸、（3）H - 胸腺嘧啶和（14）C - 尿苷掺入培养的大鼠肾片段的DNA中。虽然生长中的大鼠肾脏提取物无兴奋作用，但当与单侧肾切除大鼠的血清联合使用时，它们会使同位素掺入DNA的量相对增加——比单独使用血清时增加得更多。将（14）C - 尿苷掺入培养的大鼠肾片段的RNA中时也发现了上述情况。单侧肾切除大鼠的血清不能刺激大鼠肝切片中的DNA合成，但在存在生长中的肾脏提取物时则可以。血清和提取物中的促肾生长因子似乎不是通过稀释同位素、增强转运或影响肾细胞的整体代谢来发挥作用的。上述血清和肝脏因子可能在肾脏代偿性生长中起作用。
