Guidotti T L, Abraham J L, DeNee P B, Smith J R
Arch Environ Health. 1978 May-Jun;33(3):117-24. doi: 10.1080/00039896.1978.10667320.
Study of lung tissue from necropsy of a 58-year-old arc welder with arc welders' pneumoconiosis, confirmed by history, chest radiography, and pathology, demonstrates the versatility and usefulness of new techniques in scanning electron microscopy (SEM). Secondary electron imaging, the most familiar SEM mode, showed heavy cellular infiltrates in alveoli, the interstitium, and within the interstices of loose whorled fibrotic nodules. Backscattered electron imaging, in which contrast is proportional to elemental atomic number, revealed intracellular metal particles not otherwise visible. Microprobe analysis, energy-dispersive x-ray spectrometry, mapped elemeental iron over the particle image and identified traces of silicon in the whorled nodules. Arc welders' pneumoconiosis appears to be more than a benign siderosis resulting from particulate iron deposition. Simultaneous exposure to other components of welding fumes may alter the pathologic picture, inducing a more complicated fibrotic reaction. The more recently developed advanced techniques of SEM are well suited to the study of pneumoconioses and other problems of heterogenous tissue and mixed chemical systems.
对一名58岁患有电焊工尘肺的电弧焊工进行尸检，其肺组织研究经病史、胸部X线摄影及病理学证实。该研究展示了扫描电子显微镜（SEM）新技术的多样性和实用性。二次电子成像作为最常见的SEM模式，显示肺泡、间质以及疏松涡状纤维化结节间隙中有大量细胞浸润。背散射电子成像中，对比度与元素原子序数成正比，揭示了细胞内金属颗粒，而在其他情况下这些颗粒不可见。微探针分析、能量色散X射线光谱法在颗粒图像上绘制了元素铁，并在涡状结节中鉴定出硅的痕迹。电焊工尘肺似乎不仅仅是由颗粒状铁沉积导致的良性铁尘肺。同时接触焊接烟雾的其他成分可能会改变病理状况，引发更复杂的纤维化反应。SEM最近开发的先进技术非常适合研究尘肺以及其他异质性组织和混合化学系统问题。
