大鼠脑微血管单胺氧化酶和儿茶酚-O-甲基转移酶的研究。

Studies on the monoamine oxidase and catechol-O-methyltransferase of the rat cerebral microvessels.

作者信息

Lai F M, Spector S

出版信息

Arch Int Pharmacodyn Ther. 1978 Jun;233(2):227-34.

Abstract

The rat cerebral microvessels were isolated and assayed both for monoamine oxidase (MAO) and catechol-o-methyltransferase (COMT) activities. Cerebral microvessels and brain filtrate were found to contain both forms (A & B) of MAO. On a per mg protein basis, the cerebral microvessels contained the greatest activity of MAO and COMT than that of brain filtrate and mesenteric artery. Chemical sympathectomy using 6-hydroxydopamine neither decreased total MAO activity, nor the A form of the enzyme, nor did it have any effect on COMT activity in the brain microvessels. These results suggest that COMT and both forms of MAO in the rat brain microvessels are extraneuronal in origin.

摘要

分离大鼠脑微血管并检测其单胺氧化酶（MAO）和儿茶酚-O-甲基转移酶（COMT）活性。发现脑微血管和脑滤液中均含有MAO的两种形式（A和B）。以每毫克蛋白质计算，脑微血管中MAO和COMT的活性高于脑滤液和肠系膜动脉。使用6-羟基多巴胺进行化学交感神经切除术既未降低MAO的总活性，也未降低该酶的A形式，对脑微血管中的COMT活性也没有任何影响。这些结果表明，大鼠脑微血管中的COMT和MAO的两种形式均起源于神经外。