Hanson A N, Bentley J P
Anal Biochem. 1983 Apr 1;130(1):32-40. doi: 10.1016/0003-2697(83)90646-2.
A method is described whereby the ratio of the major interstitial collagens (Types I and III) can be measured in biopsy specimens of human tissue weighing as little as 25 mg. Marker peptides are solubilized from the tissue by digestion with cyanogen bromide. These peptides which are not known to be involved in collagen crosslinking are isolated and quantified by a combination of carboxymethyl-cellulose chromatography and polyacrylamide gel electrophoresis. The peptides used are alpha 1(I)-CB7 and alpha 1(III)-CB5. The use of the method is illustrated by analyzing the collagen type ratio in small specimens of tendon, aorta, and vena cava.
本文描述了一种方法，通过该方法可以在重量仅为25毫克的人体组织活检标本中测量主要间质胶原蛋白（I型和III型）的比例。通过用溴化氰消化从组织中溶解标记肽。这些未知参与胶原蛋白交联的肽通过羧甲基纤维素色谱法和聚丙烯酰胺凝胶电泳相结合的方法进行分离和定量。所使用的肽是α1(I)-CB7和α1(III)-CB5。通过分析肌腱、主动脉和腔静脉小标本中的胶原类型比例来说明该方法的应用。
