一种用于聚丙烯酰胺凝胶定量分析的高空间分辨率激光束密度计。

A high-spatial-resolution laser beam densitometer for quantitative analysis of polyacrylamide gels.

作者信息

Brayden J E, Halpern W

出版信息

Anal Biochem. 1983 Apr 1;130(1):9-13. doi: 10.1016/0003-2697(83)90642-5.

Abstract

Quantitative analysis of proteins separated on polyacrylamide gels requires the use of an accurate, sensitive scanning device. An easily constructed, high-resolution laser beam densitometer is described in this report. The apparatus was built from readily obtainable but precision optical, electronic, and mechanical components. It was designed for and successfully applied to analysis of microslab gels, but also can be used for scanning of cylindrical gels, autoradiographs, and, with simple modification, larger slab gels.

摘要

对在聚丙烯酰胺凝胶上分离的蛋白质进行定量分析需要使用精确、灵敏的扫描设备。本报告介绍了一种易于构建的高分辨率激光束密度计。该仪器由易于获得但精密的光学、电子和机械部件制成。它专为微板凝胶分析而设计并成功应用，也可用于扫描柱状凝胶、放射自显影片，经过简单改装还可用于扫描更大的板状凝胶。