一种从蛋白质溶液中去除去污剂的快速方法。
A rapid method for removal of detergents from protein solution.
作者信息
Horigome T, Sugano H
出版信息
Anal Biochem. 1983 Apr 15;130(2):393-6. doi: 10.1016/0003-2697(83)90605-x.
PMID:6869826
Abstract
A simple and rapid technique is described for the removal of Triton X-100, deoxycholate, and cholate from protein solutions. The method involves a 2-min centrifugation of the sample on a Bio-Beads SM-2 bed prepared in a microcentrifuge tube and is suitable for multiple assays of 0.05- to 0.45-ml samples. Another advantage of this method is the high recovery of proteins without dilution of the sample.
摘要
本文描述了一种从蛋白质溶液中去除Triton X-100、脱氧胆酸盐和胆酸盐的简单快速技术。该方法包括将样品在微量离心管中制备的Bio-Beads SM-2柱上离心2分钟，适用于0.05至0.45毫升样品的多次检测。该方法的另一个优点是蛋白质回收率高，且无需稀释样品。
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