一项关于GDP与从棕色脂肪组织中纯化的产热素蛋白结合的研究。
A study of GDP binding to purified thermogenin protein from brown adipose tissue.
作者信息
French R R, Gore M G, York D A
机构信息
Department of Biochemistry, School of Biochemical and Physiological Sciences, University of Southampton, U.K.
出版信息
Biochem J. 1988 Apr 15;251(2):385-9. doi: 10.1042/bj2510385.
Abstract
- The binding of GDP to purified thermogenin protein was studied by using fluorescence spectroscopy and equilibrium dialysis. 2. GDP binding to thermogenin diminished fluorescence emission in a concentration-dependent manner that exhibited saturation. 3. Kd values for binding of nucleoside di- and tri-phosphates were lower than those for nucleoside monophosphates. 4. The GDP-induced fluorescence quenching was decreased by increasing pH, but the apparent Kd was unaltered by pH changes. 5. Equilibrium dialysis showed a Kd change from 3 to 6 microM when the pH was increased from 6.6 to 8.5. 6. The apparent pK of the fluorescence changes induced by pH (8.3) was identical with the apparent pK of the GDP-binding response. 7. The data are consistent with the existence of protonated and non-protonated forms of thermogenin protein that both bind GDP.
摘要
- 利用荧光光谱法和平衡透析法研究了GDP与纯化的产热素蛋白的结合。2. GDP与产热素的结合以浓度依赖的方式降低荧光发射，呈现出饱和现象。3. 核苷二磷酸和三磷酸结合的Kd值低于核苷单磷酸的Kd值。4. 增加pH值可降低GDP诱导的荧光猝灭，但表观Kd不受pH变化影响。5. 平衡透析显示，当pH从6.6增加到8.5时，Kd从3微摩尔变为6微摩尔。6. pH诱导的荧光变化的表观pK（8.3）与GDP结合反应的表观pK相同。7. 这些数据与产热素蛋白存在质子化和非质子化形式且都能结合GDP的情况一致。
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本文引用的文献
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